⭐ ONLINE COURSE DESCRIPTION (3-CLASS MODULE)

MASTERING HEMATOLOGY BASICS – From Bench Skills to Flow Cytometry Interpretation

Instructor: Dr. Deepa

• Hemato-Cytopathologist • Founder, Dr. Deepa’s Learning Lounge

🔵 CLASS 1: Foundations in Coagulation & Hemoglobin Analysis

This session builds the essential groundwork for every hematology learner.
You will learn:

🔬 LAB EXERCISE: PT/APTT – Interpretation & Troubleshooting

• Step-wise demonstration of PT & APTT assay
• Understanding extrinsic, intrinsic & common pathways
• Pre-analytical errors, sample handling, INR calculation
• Clinically relevant interpretation made simple
• Frequently asked viva questions for exam readiness

🧬 HPLC – Basic Interpretation & Key Hemoglobinopathies

• How to read HPLC chromatograms
• Normal retention times explained
• HbA2, HbF patterns & thalassemia screening
• Differentiating common variants (HbS, HbE, δβ-thal, HPFH)

🖼 SPOTTERS (With 10-20 Viva Questions Each)

🔵 CLASS 2: Staining Masterclass, Flow Cytometry Basics & Acute Leukemia

🎨 LAB EXERCISE: Staining Techniques (Romanowsky Series)

• Leishman, Giemsa, Wright, Field stain – principles & protocols
• Common staining artifacts and troubleshooting
• How pH, fixation & smear thickness affect morphology
• Viva questions for practical examinations

🔍 BASIC FLOW CYTOMETRY INTERPRETATION – Acute Leukemia

A beginner-friendly introduction to immunophenotyping.
Learn:

• Understanding FSC/SSC plots
• Gating strategies for blasts
• Distinguishing AML, B-ALL, T-ALL
• Acute leukemia algorithms explained step-by-step

🖼 SPOTTERS (With 1-20 viva Questions each)

🔵 CLASS 3: Advanced Coagulation, CLPD Flow Cytometry & Essential Calculations

🧪 LAB EXERCISE: Factor VIII One-Stage Assay

• Principle & stepwise dilution method
• How to prepare the calibration curve
• Graph plotting for % factor activity
• Understanding assay limitations

🧪 BASICS OF INHIBITOR ASSAY (Bethesda Method)

• What is a Bethesda Unit?
• How inhibitors are measured
• Step-by-step demonstration with interpretation pearls

🔬 FLOW CYTOMETRY IN CLPD (Chronic Lymphoproliferative Disorders)

• CLL, Mantle Cell Lymphoma, Follicular Lymphoma, Hairy Cell Leukemia

🧮 CRITICAL CALCULATIONS EVERY HEMATOLOGIST MUST KNOW

• CD34 enumeration for stem cell transplantation
• TNC calculations for cord blood/bone marrow units